- 1). Attach one end of the hose to the cooler's drain hole and the other end to the flow regulator that is connected to the carbon dioxide tank. If your cooler doesn't have a drain hole, drill out a hole and stick the hose in it.
- 2). Add a window to your chamber. Use a jigsaw to drill through the cooler. Make an opening for a window. Cut a piece of plexiglass to fit. Attach it to the opening with epoxy.
- 3). Drill a vent hole near the top of the chamber to allow some of the CO2 to escape. The chamber should not be airtight. A vent hole will let the air out, but not the CO2.
- 4). Work in a well-ventilated area to prevent your exposure to the gas. Place a small cage containing the animal in the CO2 chamber. Close the chamber. Set the flow rate so that it displaces 20 percent of the oxygen each minute.
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